发明名称 |
TAGGED MICROPARTICLE COMPOSITIONS AND METHODS |
摘要 |
Compositions and methods are disclosed for detecting multiple target analytes in a sample using microparticles having molecular tags attached by cleavable linkages. Generally, an assay mixture is formed comprising a sample and a reagent comprising multiple such microparticles under conditions that permit stable complexes to form between binding moieties on the surfaces of the microparticles and the analytes. In one aspect of the invention, the a second binding composition is added so that complexes form among the microparticle-bound binding moieties, the analytes, and second binding moieties of the second binding composition. Such second binding moieties have cleavage-inducing moieties attached that upon activation cause the cleavage of the cleavable linkages and the release of molecular tags. Released molecular tags are separated and the presence and/or amount of the target analytes are determined based on the analysis of the released and separated molecular tags.
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申请公布号 |
CA2465594(C) |
申请公布日期 |
2012.02.07 |
申请号 |
CA20022465594 |
申请日期 |
2002.11.08 |
申请人 |
ACLARA BIOSCIENCES INC. |
发明人 |
DEHLINGER, PETER;SINGH, SHARAT;KIRAKOSSIAN, HRAIR |
分类号 |
G01N33/543;C07B61/00;C07D405/12;C07D493/10;C07D495/04;G01N21/00;G01N21/76;G01N27/447;G01N30/88;G01N33/00;G01N33/53;G01N33/533;G01N33/536;G01N33/537;G01N33/545;G01N33/551;G01N33/553;G01N33/554 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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