发明名称 |
METHODS FOR ANTIBODY ENGINEERING |
摘要 |
The invention provides a method for identifying positions of an antibody that can be modified without significantly reducing the binding activity of the antibody. In many embodiments, the method involves identifying a substitutable position in a parent antibody by comparing its amino acid sequence to the amino acid sequences of a number of related antibodies that each bind to the same antigen as the parent antibody. The amino acid at the substitutable position may be substituted for a different amino acid without significantly affecting the activity of the antibody. The subject methods may be employed to change the amino acid sequence of a CDR without significantly reducing the affinity of the antibody of the antibody, in humanization methods, or in other antibody engineering methods. The invention finds use in a variety of therapeutic, diagnostic and research applications. |
申请公布号 |
US2012003671(A1) |
申请公布日期 |
2012.01.05 |
申请号 |
US201113100089 |
申请日期 |
2011.05.03 |
申请人 |
COUTO FERNANDO JOSE REBELO DO;HENDRICKS KRISTIN B.;WALLACE S. ELLEN;YU GUO-LIANG |
发明人 |
COUTO FERNANDO JOSE REBELO DO;HENDRICKS KRISTIN B.;WALLACE S. ELLEN;YU GUO-LIANG |
分类号 |
G01N33/566;C07K16/00;C12P21/00 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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