USE OF DOUBLE STRANDED RNA TO INCREASE THE EFFICIENCY OF TARGETED GENE ALTERATION IN PLANT PROTOPLASTS

摘要

Method for targeted gene alteration in protoplasts of plant cells comprising the steps of transiently transfecting the protoplasts with a dsRNA that preferably targets plant MMR mRNA; and a mutagenic nucleobase. The transfection may be simultaneously or subsequently and the gene can be any gene functional in the mismatch repair system.