发明名称 |
CONFORMATIONAL PROBES AND METHODS FOR SEQUENCING NUCLEIC ACIDS |
摘要 |
This disclosure provides a method of determining a sequence of nucleotides for a nucleic acid template. The method can include the steps of contacting the nucleic acid template with a conformationally labeled polymerase and at least four different nucleotide species under conditions wherein the conformationally labeled polymerase catalyzes sequential addition of the nucleotide species to form a nucleic acid complement of the nucleic acid template, wherein the sequential addition of each different nucleotide species produces a conformational signal change from the conformationally labeled polymerase and wherein the rate or time duration for the conformational signal change is distinguishable for each different nucleotide species; detecting a series of changes in the signal from the conformationally labeled polymerase under the conditions; and determining the rates or time durations for the changes in the signal, thereby determining the sequence of nucleotides for the nucleic acid template.
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申请公布号 |
US2011312529(A1) |
申请公布日期 |
2011.12.22 |
申请号 |
US201113162325 |
申请日期 |
2011.06.16 |
申请人 |
HE MOLLY;CHEN CHENG-YAO;KOOL ERIC;RONAGHI MOSTAFA;PREVITE MICHAEL;PANTOJA RIGO;ILLUMINA, INC. |
发明人 |
HE MOLLY;CHEN CHENG-YAO;KOOL ERIC;RONAGHI MOSTAFA;PREVITE MICHAEL;PANTOJA RIGO |
分类号 |
C40B30/04;C40B30/00 |
主分类号 |
C40B30/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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