| 摘要 |
<p>Non-human vertebrates (e.g. a mouse or rat) or cells are disclosed comprising an antibody heavy chain transgene, wherein the transgene comprises one or more human VH , D or JH gene segments operably connected upstream of a constant region gene so that the transgene can undergo VDJ recombination in vivo to produce an antibody gene comprising a rearranged VDJC encoding an antibody heavy chain having a human variable region and a constant region, or wherein the transgene comprises a rearranged VDJ encoding a human variable region operably connected upstream of a constant region gene, the genome further comprising a human VpreB gene capable of expressing human a VpreB. Embodiments also provide for species-matching of the entire surrogate light chain for efficient pairing with chimaeric heavy chains during B-cell development in vivo in a non-human transgenic vertebrate. The invention is directed towards improving the production of chimaeric antibodies.</p> |
