| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide nucleic acid template preparation for real-time PCR. <P>SOLUTION: The invention is related to a novel reagent formulation for the efficient preparation of a nucleic acid template from cells for high throughput real-time PCR analysis. The reagent permits rapid cell lysis and template preparation without the need for template purification and additional isolation. The reagent therefore dramatically improves throughput of real-time PCR analysis while at the same time permitting the rapid and sensitive real-time Catacleave PCR detection of a single molecule of nucleic acid template in as little as about 35 cycles of PCR amplification. <P>COPYRIGHT: (C)2011,JPO&INPIT |
