| 摘要 |
The present invention relates to a method to recover an intracellular polyhydroxyalkanoates (PHA). The method includes the steps of (a) separating culture medium containing PHA-rich cells using centrifugation, (b) rinsing pellet obtained from step (a) with distilled water, (c) freeze- drying the pellet obtained in step (b), (d) adding distilled water to the freeze-dried sample, (e) separating non-PHA cellular material (NPCM) from PHA granules, (f) washing the pellet containing PHA from step (e) by distilled water and centrifuging for producing final pellet, and (g) freeze-drying the pellet produced from step (f). |
| 申请人 |
UNIVERSITI PUTRA MALAYSIA;HASSAN, MOHD, ALI;MOHAMMADI, MITRA;AB RAZAK, NOR, ASMA;CHONG, MEI, LING;ABD AZIZ, SURAINI;ABDULLAH, AMIRUL, AL-ASHRAF;CHE MAN, HASFALINA;ANIS, SITI, NOR, SYAIRAH;PHANG, LAI, YEE;SHIRAI, YOSHIHITO;WAN YUNUS, WAN, MD, ZIN |
发明人 |
HASSAN, MOHD, ALI;MOHAMMADI, MITRA;AB RAZAK, NOR, ASMA;CHONG, MEI, LING;ABD AZIZ, SURAINI;ABDULLAH, AMIRUL, AL-ASHRAF;CHE MAN, HASFALINA;ANIS, SITI, NOR, SYAIRAH;PHANG, LAI, YEE;SHIRAI, YOSHIHITO;WAN YUNUS, WAN, MD, ZIN |
