| 摘要 |
Provided is a method for determining ovarian activity in a mammal by means of estrogen metabolite and progesterone metabolite measurements, comprising: obtaining two or more urine samples from a mammalian female subject, wherein the two or more urine samples are collected at intervals of time from between about 2 to about 4 days, from about 2 to about 5 days, from about 2 to about 6 days, from about 2 to about 7 days, from about 2 to about 8 days, from about 2 to about 9 days, from about 2 to about 10 days, from about 2 to about 12 days, from about 2 to about 15 days, from about 2 to about 20 days, from about 2 to about 25 days, from about 2 to about 28, or from about 2 to about 30 days and further wherein each said urine sample is prepared by collecting urine from said female subject over a 3-12 hour time period and normalizing the urine sample to a constant volume per unit time; contacting an amount of said sample with a solid phase capture element, a first binding protein capable of binding an estrogen metabolite and a second binding protein capable of binding a progesterone metabolite; determining the excretion rate of said estrogen metabolite and said progesterone metabolite in said sample; and determining the ovarian activity of said female subject based upon the excretion rates of said estrogen metabolite and said progesterone metabolite, wherein the coefficient of variation of said determination is less than 10%, and wherein: a statistically significant rise in the estrogen metabolite excretion rate indicates the beginning of a fertility phase; an ovulation cycle peak in the estrogen metabolite excretion rate indicates the time frame for optimal fertility; biochemical proof of ovulation is provided by an increase in the rate of progesterone metabolite excretion above a PdG ovulation threshold, or a rate of progesterone metabolite excretion above a PdG fertility threshold marks an end of the fertility phase. Combined analysis of estrogen and progesterone metabolite measurements and display of results can also be made remotely by a communication network.
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