发明名称 METHOD FOR EXTRACTING NUCLEIC ACID OF BROWN ALGAE, METHOD FOR DETERMINING SPECIES OF BROWN ALGAE AND KIT FOR EXTRACTING NUCLEIC ACID OF BROWN ALGAE
摘要 PROBLEM TO BE SOLVED: To provide a method for easily and efficiently extracting a nucleic acid having high quality from brown algae, a method for determining species of the brown algae based on the extracted nucleic acid, and a kit for extracting the nucleic acid from the brown algae. SOLUTION: The method for extracting the nucleic acid from brown algae comprises a step to decompose a viscous polysaccharide of the brown algae using a polysaccharide decomposition enzyme extracted from a marine mollusk, a step to suppress gel-formation of the viscous polysaccharide of the brown algae by using a chelating agent, and a step to extract the nucleic acid of the brown algae by using a proteolytic enzyme. The nucleic acid having high quality is easily and efficiently extracted from the brown algae by using the method for extracting the nucleic acid of the brown algae and the kit for extracting the nucleic acid of the brown algae. The species of the brown algae can be surely determined by the species-determining method for the brown algae using the extracted nucleic acid. COPYRIGHT: (C)2011,JPO&INPIT
申请公布号 JP2011160755(A) 申请公布日期 2011.08.25
申请号 JP20100029136 申请日期 2010.02.12
申请人 HOKKAIDO UNIV;HAKODATE CHIIKI SANGYO SHINKO ZAIDAN;HOKKAIDO RESEARCH ORGANIZATION 发明人 INOUE AKIRA;SHIMIZU KENJI;YASOGAWA DAISUKE
分类号 C12Q1/68;C12N15/09 主分类号 C12Q1/68
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