RECOMBINANT CO-EXPRESSION OF VITAMIN K EPOXIDE REDUCTASE SUBUNIT 1 TO IMPROVE VITAMIN K DEPENDENT PROTEIN EXPRESSION

摘要

PROBLEM TO BE SOLVED: To provide new systems and methods for improving the productivity of (particularly recombinant) VKD protein expression via co-expression of VKORC1.

SOLUTION: A host organism contains recombinant nucleic acids coding for the vitamin K reductase complex subunit 1 (VKORC1) and recombinant nucleic acids coding for a vitamin K dependent (VKD) protein, wherein both the recombinant VKORC1 and the recombinant VKD protein are expressed in the host organism. Further, there are provided a cell culture system comprising cells which contain the recombinant nucleic acids and methods for improving the productivity of recombinant VKD protein expression in a host organism being cultured in suitable systems.

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