发明名称 |
ASSAY FOR IDENTIFYING AGENTS THAT MODULATE EPIGENETIC SILENCING, AND AGENTS IDENTIFIED THEREBY |
摘要 |
A high throughput RNAi-based assay for identify factors involved in maintaining epigenetic silencing is disclosed. The assay measures reactivation of a silent reporter gene in cells, resulting from RNAi-based knockdown in target mRNA. RNAi-based screening of these silent reporter cells has identified known enzymes that place or remove epigenetic marks on histones, as well as non-enzymatic proteins that function in silencing or in transfer of marks during S-phase. In addition, the screen has been used to identify a number of novel gene products involved in epigenetic silencing, which are also disclosed. |
申请公布号 |
US2011172107(A1) |
申请公布日期 |
2011.07.14 |
申请号 |
US20090736702 |
申请日期 |
2009.04.30 |
申请人 |
FOX CHASE CANCER CENTER |
发明人 |
KATZ RICHARD A.;SKALKA ANNA MARIE |
分类号 |
C12Q1/68;C07K14/47;C12N5/071;C12N9/00;C12N9/02;C12N9/10;C12N9/78;C40B30/00 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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