发明名称 |
RODENT MODEL FOR THE FAST IDENTIFICATION OF PHARMACEUTICAL ACTIVE COMPOUNDS IN VIVO |
摘要 |
In a first aspect the present invention provides a tumor cell line stably transfected with an expression vector containing a reporter gene, preferably a fluorescent protein, operably linked to a promotor that also controls expression of a protein that is associated with tumor regression, stabilization of tumor growth or inhibition of metastatic growth, characterized in that said cell line is capable to form a tumor when implanted or injected into the non-human animal. Compared to the traditional in vivo models, the present invention differs in that the reporter gene is not constitutively expressed, but only after exposure to a test compound that results in the expression of a protein or enzyme associated with tumor regression, stabilization of tumor growth or inhibition of metastatic growth. Only when a compound to be tested got into circulation and infiltrated the tumor it may generate the reporter signal, provided it promotes the expression of a protein associated with tumor regression and the promoter of said protein is operably linked to the reporter gene. |
申请公布号 |
EP1601775(B1) |
申请公布日期 |
2011.07.13 |
申请号 |
EP20040716577 |
申请日期 |
2004.03.03 |
申请人 |
JANSSEN PHARMACEUTICA NV |
发明人 |
BELIEN, ANN, TRUDO, JOSEE;ARTS, JANINE;MARIEN, ANN, ODETTE, ADOLF;VALCKX, ANNEMIE, FRANCINE |
分类号 |
C12N15/85;A01K67/027;A61K49/00;C07K14/47;C12N;C12N5/10;C12Q1/68;G01N33/50 |
主分类号 |
C12N15/85 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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