| 摘要 |
FIELD: medicine. ^ SUBSTANCE: NA hybridisation is enabled by (a) heating of a hybridisation solution containing NA to temperature equal to or above than NA denaturation temperature that is followed with (b) cooling of the heated hybridisation solution to temperature whereat NA is hybridised with probes immobilised on a solid phase, and (c) hybridisation of NA contained in the cooled hybridisation solution with the probes immobilised on the solid phase at hybridisation temperature. According to the method, hybridised nucleic acids are transferred between denaturation, cooling and hybridisation zones; flow rate of the hybridisation solution with NA provides heating of the hybridisation solution at the stage (a) to temperature equal to or above than denaturation temperature with total heating time greater than denaturation time summerised with diffuse NA divergence time, and cooling at the stages (b) and (c) to hybridisation temperature with general cooling time at the stages (b) and (c) smaller than NA renaturation time. ^ EFFECT: method is completely compatible with a widespread microchip technology and enables hybridisation of both strands of two-strand DNA without preliminary run of one-strand DNA from two-strand one. ^ 86 cl, 10 dwg, 2 ex |
