The present invention relates to improved processes for purifying polypeptides of interest by increasing the amount of a polypeptide of interest bound to an ion- exchange matrix relative to the amount of one or more impurities bound to the ion-exchange matrix. This effect is achieved by adding a chemical compound in the process which by also binding to the ion-exchange matrix due to a change that is opposite to the change of the ion-exchangematrix,reduces the binding of impurities more than the binding of the polypeptide of interest.
申请公布号
CA2783207(A1)
申请公布日期
2011.06.23
申请号
CA20102783207
申请日期
2010.12.15
申请人
CSL LTD.
发明人
CHARLTON, ADAM;NAPOLI, MARK;SMRDELJ, PAUL;STOWERS, ANTHONY;PIRZAS, VICKY;SCHROEDER, MAGNUS