| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a method of rapidly extracting RNA by membrane, especially the method of extracting RNA for applying to an intraoperative diagnosis. <P>SOLUTION: There is provided a method of extracting RNA from biological systems (cells, cell fragments, organelles, tissues, organs or organisms). The method is that a solution containing RNA is contacted with a substrate to which RNA can bind. RNA is withdrawn from the substrate by applying negative pressure. No centrifugation step exceeds thirty seconds. Preferably, the RNA is diluted prior to filtration and one or more wash steps can be used to remove interferents. In one such method, except for DNA shearing and drying steps, centrifugation is not undertaken during the extraction step. <P>COPYRIGHT: (C)2011,JPO&INPIT |
