ZUSAMMENSETZUNGEN UND VERFAHREN ZUR BEARBEITUNG UND AMPLIFIKATION VON DNA MIT VERWENDUNG MEHRERER ENZYME IN EINER EINZIGEN REAKTION

摘要

The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome.