| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide methods for purifying one or more cells. <P>SOLUTION: The methods for purifying one or more cells based on the level of one or more products secreted by the cells is provided. The method involves: (a) contacting a plurality of cells, immobilized in proximity to a capture matrix, with an agent that selectively binds to the product; (b) illuminating a population of the cells; (c) detecting two or more properties of light directed from a frame, wherein a first property of light identifies substantially all cells of the population, and the second property of light identifies product localized to the capture matrix; (d) locating (i) substantially all cells of the population with reference to the detected first property of light, and (ii) one or more selected cells with reference to the detected second property of light; and (e) irradiating the non-selected cells, whereby one or more selected cells having a desired product secretion profile are purified. <P>COPYRIGHT: (C)2011,JPO&INPIT</p> |
