Mutated netrin 1, fragments thereof and uses thereof as drugs

摘要

(Mutant) netrin-4 protein (I) comprising at least 230 amino acids as in SEQ ID. NOs 522 and 524, its fragment, derivative or homologue, is new. (Mutant) netrine-4 protein (I) comprising or contains one of the following sequences; SEQ ID NO. 522 or 524, a fragment of 522/524 with anti-angiogenic activity and/or perictye activation activity, where the fragment comprises between 40 and 450 amino acids, preferably between 40 and 230 amino acids and is represented by SEQ ID NO: 2q, q variant of 187 to 248 or by SEQ ID NO 526 or 528. Also included are the above sequences with substitutions, suppression or addition of one or more amino acids providing that the sequences has anti-angiogenic or perictye activation activity. Active homologues are also included having at least approximiately 50 % sequence identity with the region between amino acids 261 to 515 in SEQ ID NO: 522. Sequence information is not fully defined in the specification. Independent claims are also included for: (1) nucleotide sequence (II) encoding the above proteins of (I); (2) recombinant vector (IV), plasmid, cosmid, phage or DNA virus comprising (II) comprising all necessary elements for expression in a suitable host cell for expression of (I); (3) host cell (IV), chosen from bacteria, viruses, yeast, mushrooms, plants or mammalian cells, transformed by (IV); (4) antibody (V), specific for any of (I); (5) anti-idiotypic antibody (VI) specific for any of (I); ACTIVITY : Cytostatic; Opthalmological; Antiangiogenic; Immunosuppressive; Vasotropic; Hypotensive; Antiarthritic; Cardiant; Anorectic; Cerebroprotective; Dermatological; Antiarteriosclerotic. No biological data is given. MECHANISM OF ACTION : None given.