发明名称 |
METHOD OF OPTIMIZING CODON USAGE THROUGH DNA SHUFFLING |
摘要 |
The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.
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申请公布号 |
US2011111413(A1) |
申请公布日期 |
2011.05.12 |
申请号 |
US20100952209 |
申请日期 |
2010.11.23 |
申请人 |
PADGETT HAL S;LINDBO JOHN A;FITZMAURICE WAYNE P;VAEWHONGS ANDREW A |
发明人 |
PADGETT HAL S.;LINDBO JOHN A.;FITZMAURICE WAYNE P.;VAEWHONGS ANDREW A. |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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