发明名称 |
METHOD, AGENT, AND KIT FOR ISOLATING NUCLEIC ACIDS |
摘要 |
A method for isolating nucleic acids is provided. The method includes providing a biological sample containing at least one nucleic acid, and mixing the biological sample with an isolating agent under a suitable condition to isolate the nucleic acids from the biological sample in single step, wherein the isolating agent contains 1-40 wt % of PEG and/or more than 30 wt % of low molecular weight alcohol, a salt, and a detergent. Isolated nucleic acids are bound to a solid support by changes in the solubility of nucleic acids. Additionally, the present invention further provides an isolating agent and kit for isolating nucleic acids. |
申请公布号 |
US2011097782(A1) |
申请公布日期 |
2011.04.28 |
申请号 |
US20100905777 |
申请日期 |
2010.10.15 |
申请人 |
INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE |
发明人 |
JIANG PEI-SHIN;WU KUN-CHAN;SU YU-TING;LIN CHIA-YUN;SU SIOU-CING;LIN YUH-JIUAN |
分类号 |
C12N9/50;C07H21/00;C09K3/00;C12N1/08 |
主分类号 |
C12N9/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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