IN VITRO GENERATION OF CYTOTOXIC T CELLS BY USING CXCR3+CCR4-CD4+ T CELLS AND DENDRITIC CELL

摘要

PURPOSE: An in vitro method for inducing cytotoxic Th1 cells which express CXCR3+CCR4- and dendritic cells is provided to be applied to adoptive T cell immunotherapy. CONSTITUTION: A composition for maturing dendritic cells contains Th1 cells which express CXCR3high/CCR4low. The dendritic cells are matured by culturing the Th1 cells and immature dendritic cells. A pharmaceutical composition for treating tumor or viral diseases contains the tumor or viral antigen-sensitized dendritic cells; and the Th1 cells. The tumor antigen is Wilm's Tumor antigen 1 (WT-1) peptide with an amino acid sequence of sequence number 1. The viral antigen is cytomegalovirus(CMV) peptide with an amino acid sequence of sequence number 2.