<p>A method of protein engineering is provided wherein a searchable computer database is created comprising entries in the form of descriptions of a location and orientation in 3D space of side chains of the constituent amino acid residues of a framework protein. A query is created which corresponds to a description of a location and orientation in 3D space of respective side chains of amino acid residues of a sample protein. The location and orientation in 3D space of constituent side-chains is preferably described as a Calpha Cbeta vector. The query is used to search the database and thereby identify a hit which corresponds to a framework protein having structural similarity with said sample protein. Framework protein "hits" so identified may be suitable candidates for further modification. A particular advantage of the present invention is that a modified framework protein may display one or more desired characteristics, such as a function similar to or inhibitory of the sample protein.</p>
申请公布号
EP1123316(B1)
申请公布日期
2011.03.02
申请号
EP19990970666
申请日期
1999.10.21
申请人
THE UNIVERSITY OF QUEENSLAND
发明人
SMYTHE, MARK, LESLIE;DOOLEY, MICHAEL, JOHN;ANDREWS, PETER, RONALD