A FACILE SYSTEM FOR ENCODING UNNATURAL AMINO ACIDS IN MAMMALIAN CELLS

摘要

This invention provides translation system components functional in both eubacterial and eukaryotic environments. The translation system components, such as aminoacyl-tRNA synthetases and tRNAs derived from Methanosarcina species are capable of charging unnatural amino acids, and can be shuttled from enterobacteria to mammalian cells.