USE OF POROUS MEMBRANE TO SUPPORT DEVELOPING CONIFER SOMATIC EMBRYOS

摘要

The present invention provides methods for developing conifer cotyledonary somatic embryos. In some embodiments, the methods of the invention include the step of culturing conifer pre-cotyledonary somatic embryos on a porous membrane, that is at least intermittently contacted with liquid development medium, for a period of time sufficient to produce conifer, cotyledonary, somatic embryos from the pre- cotyledonary somatic embryos.