发明名称 |
Bioluminogenic assay system for measuring beta-lactamase activity |
摘要 |
A bioluminogenic assay system including: providing a bioluminogenic substrate incorporating a beta-lactam antibiotic, a bioluminescence initiating compound, and a chemical linkage joining the beta-lactam antibiotic to the bioluminescence initiating compound; exposing the bioluminogenic substrate to a beta-lactamase enzyme that catalyzes the release of the bioluminescence initiating compound from the bioluminogenic substrate; co-exposing the bioluminogenic substrate to a bioluminescence indicator reaction that employs the bioluminescence initiating compound as a substrate; and detecting a light from the bioluminescence indicator reaction as a measure of the activity of the beta-lactamase enzyme.
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申请公布号 |
US7867758(B2) |
申请公布日期 |
2011.01.11 |
申请号 |
US20080060864 |
申请日期 |
2008.04.01 |
申请人 |
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
RAO JIANGHONG;SO MIN-KYUNG;YAO HEQUAN |
分类号 |
C12M1/00;C12Q1/34 |
主分类号 |
C12M1/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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