发明名称 |
METHOD FOR PREPARING SINGLE CELL-DERIVED GENOME LIBRARY OF RARE MICROORGANISM OR NOVEL MICROORGANISM BASED ON A TECHNIQUE FOR REMOVAL OF DOMINANT SPECIES |
摘要 |
PURPOSE: A method for preparing a single cell-derived genome library of rare microorganism or novel microorganism based on a technique for removing dominant species is provided to enable to confirm origin of a gene. CONSTITUTION: A method for preparing a single cell-derived genome library of rare microorganism or novel microorganism comprises: a step of performing microorganism group community analysis of collected sample; a step of labeling dominant or known microorganism in the community analysis; a step of removing labeled microorganism by primary FACS(Fluorescence Associated Cell Sorter) method; a step of labeling remaining microorganism group and isolating total microorganism group into single cells by secondary FACS method; a step of amplifying the genome form the single cells by MDA(multiple displacement amplification); and a step of preparing a genomic library. |
申请公布号 |
KR20110002277(A) |
申请公布日期 |
2011.01.07 |
申请号 |
KR20090059787 |
申请日期 |
2009.07.01 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY |
发明人 |
SONG, JAE JUN;CHOI, JONG HYUN;HAN, YUN JON;KIM, JOONG SU |
分类号 |
C12N15/10;C12Q1/02;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|