A diagnostic system for carrying out a nucleic acid sequence amplification and detection process

摘要

An integrated lab-on-a-chip diagnostic system for carrying out a sample preparation process on a fluid sample containing cells and/or particles is disclosed. The system comprises the following components formed on a common substrate: (a) an inlet for a fluid sample; (b) a lysis unit for lysis of cells and/or particles contained in the fluid sample; (c) a nucleic acid extraction unit for extraction of nucleic acids from the cells and/or particles contained in the fluid sample; (d) a reservoir containing a lysis fluid; and (e) a reservoir containing an eluent for removing nucleic acids collected in the nucleic acid extraction unit. The sample inlet is in fluid communication with the lysis unit, and an optional valve controls the flow of fluid therebetween. The lysis unit is in fluid communication with the nucleic acid extraction unit, and an optional valve controls the flow of fluid therebetween. The reservoir containing the lysis fluid is in fluid communication with the lysis unit and a valve controls the flow of fluid therebetween. The reservoir containing the eluent is in fluid communication with the nucleic acid extraction unit, and a valve controls the flow of fluid therebetween. The system further comprises a single pump or syringe for actuation of all liquids.