RECOMBINANT PLASMID DNA pET22b(+)/-LINKS 1, CODING PROTEIN WITH LINKS 1 PROPERTIES, AND STRAIN OF Escherichia coli BL21 (DE3)/pET22b(+)/-LINKS 1 - PRODUCENT OF PROTEIN WITH PROPERTIES OF HUMAM LINKS 1

摘要

FIELD: medicine. ^ SUBSTANCE: there is constructed recombinant plasmid protein-coding DNA pET22b(+)/""-Links 1, which contains Ndel/BamHI -fragment of DNA of pET22b(+) plasmid and artificial DNA sequence, coding Ndel/BamHI fragment, which includes synthetic gene of human water-soluble domain Links 1, containing unique sites of recognition by restriction endonucleases, which have the following coordinates: Xhol - 158, Hindlll - 173, EcoRI - 192, BamHI - 198, Ndel - 430, Bglll - 534, Mlul - 1256, Pstl - 4495. Strain - protein-producent is obtained by transformation of competent cells of Escherichia coli BL21 (DE3) with recombinant plasmid pET22b(+)/""-Links 1. ^ EFFECT: invention allows to obtain protein with structure and properties identical to structure and properties of natural water-soluble domain of human protein Links 1 in sufficient amount, measured in milligrams. ^ 2 cl, 1 tbl, 4 dwg, 4 ex