METHOD FOR ESTIMATING EFFECT ON VIVIPARITY PROGRAMMING

摘要

PROBLEM TO BE SOLVED: To provide an estimation system for viviparity programming capable of analyzing many specimens conveniently and rapidly.

SOLUTION: There is disclosed a method for measuring effect on viviparity programming comprising: bringing a microsphere comprising ES cells into contact with a sample; separating the sample from the microsphere; measuring a gene expression level by exposing a part of the microshpere to a neuralizing factor to create a neurosphere precursor and simultaneously by recovering total RNA from a part of residual microshpere; forming a neuronal cell line model in which a cell constituting the neurosphere is differentiated into a mature neurocyte by culturing the obtained neurosphere precursor in a neuralizing medium using a vessel for culturing a neurocyte; and measuring the cell morphology of the obtained neuronal cell line model.

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