发明名称 |
Process for preparing purified active monomer of bone-derived factor |
摘要 |
A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.
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申请公布号 |
US7811793(B2) |
申请公布日期 |
2010.10.12 |
申请号 |
US20030734583 |
申请日期 |
2003.12.15 |
申请人 |
BIOPHARMA GESELLSCHAFT ZUR BIOTECHNOLOGISCHEN ENTWICKLUNG VON PHARMAKA MBH |
发明人 |
ANDOU HIDETOSHI;HONDA JUN;SUGIMOTO SHUNJIRO;HOETTEN GERTRUD;BECHTOLD ROLF;POHL JENS |
分类号 |
C12P21/04;A61K38/00;C07K14/475;C07K14/51;C07K14/575;C12N1/20;C12N1/21;C12P21/06 |
主分类号 |
C12P21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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