发明名称 |
Nucleotide sequences coding for branched-chain amino acids export proteins, method for isolating them and their use |
摘要 |
Isolated polynucleotide (I) contains at least one sequence that (i) is 70% identical with a sequence that encodes at least one of 251 amino acid (aa) (3) or 108 aa (5) polypeptides; (ii) encodes a polypeptide at least 70% identical with (3) or (5); (iii) is the complement of (i) or (ii), or (iv) contains at least 15 consecutive bases from (i)-(iii). Independent claims are also included for the following: (a) protein derived from sequences of 753 bp (2) or 324 bp (4); (b) coryneform microorganisms, especially Corynebacterium, transformed with one or more (I), where these are replicative DNA; (c) production of branched-chain L-aa by fermentation of coryneform bacteria in which the brnE and/or brnF genes (or equivalent sequences) are amplified, especially overexpressed; and (d) method for isolating the brnE and/or brnF genes. |
申请公布号 |
EP1096010(B1) |
申请公布日期 |
2010.09.15 |
申请号 |
EP20000122057 |
申请日期 |
2000.10.11 |
申请人 |
EVONIK DEGUSSA GMBH;FORSCHUNGSZENTRUM JUELICH GMBH |
发明人 |
KENNERKNECHT, NICOLE;EGGELING, LOTHAR, DR.;SAHM, HERMANN, PROF.;PFEFFERLE, WALTER, DR. |
分类号 |
C12N15/09;C12N15/31;C07K14/34;C12N1/21;C12N15/10;C12N15/54;C12P13/06;C12P13/08;C12R1/15 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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