| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a fluorescent dye for a nucleic acid which has a satisfactory cell membrane permeability. Ž<P>SOLUTION: The present invention discloses the manufacture and use of the fluorescent dye used for dying a nucleic acid. The dye comprises a substituted benzazolium ring system which is linked by a methine bridge to a pyridinium or quinolinium ring system containing at least one substituent being saturated or unsaturated cyclic substituent. By virtue of distinguished fluorescent characteristic when forming a complex with the nucleic acid, the dye can be used for detecting an oligonucleotide and the nucleic acid in a cell, gel or solution. Because of the presence of the cyclic substituent, the permeability of the dye into cells and gel in a wide range is improved and the detection of the nucleic acid can be improved. The combination of the dye with an additional dye permits the analysis of cell membrane integrity, the Gram sign or the structure and function of cell. Ž<P>COPYRIGHT: (C)2010,JPO&INPIT Ž
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