| 摘要 |
<p>Stereoselective (preferably enantioselective) enzymatic reduction of ketocompounds to their corresponding chiral hydroxycompounds, comprises reducing the ketocompounds with an oxidoreductase, where: a polypeptide is used for the reduction of the ketocompounds; and the polypeptide includes the short-chain oxidoreductase and characteristics of e.g. (a) a N-terminal Rossmann-Fold GlyxxxGlyxGly, (b) a AsnAsnAlaGly-motif at position 86, (c) a catalytic triad consisting of Ser 139, Tyr 152 and Lys 156, and (d) a negatively charged amino acid residue at position 37. Stereoselective (preferably enantioselective) enzymatic reduction of ketocompounds to their corresponding chiral hydroxycompounds, comprises reducing the ketocompounds with an oxidoreductase, where: a polypeptide is used for the reduction of the ketocompounds; the polypeptide includes the short-chain oxidoreductase and characteristics of (a) a N-terminal Rossmann-Fold GlyxxxGlyxGly, (b) a AsnAsnAlaGly-motif at position 86, (c) a catalytic triad consisting of Ser 139, Tyr 152 and Lys 156, (d) a negatively charged amino acid residue at position 37 (according to the numerical system of 3alpha , 20beta -hydroxysteroid dehydrogenase), (e) a C-terminal motif in the dimerization domain [Val;Ile]-AspGly-[Gly;Ala]-Tyr-[Thr;Val;Cys]-[Ala;Thr]-[Gln;Val;Arg;Leu], (f) a (R)-alcoholdehydrogenase-signature at position 204-208, His-[Pro;Ala]-[Ile;Ala;Gln; Val]-GlyArg, (g) Val or Leu at position 160 (4 points downwards to Lys 156), and (h) Asp at position 178; and x is not defined.</p> |
