Recombinant Lol p 5, Phl p 5 and Poa p 5 allergens with reduced IgE binding but undiminished T-cell antigenicity

摘要

Disclosed is a recombinant pollen allergen variant comprising reduced numbers of IgE epitopes, which variant exhibits reduced binding capacity for IgE and/or which exhibits reduced IgE production stimulatory activity while retaining T-cell antigenicity selected from the list consisting of Lolium perene pollen allergen 5 (Lol p 5) variants carrying the mutations: a. K57A, G273A, K275A, K172N, F173L, T174A and V175A (mut 6); b. K57A, G273A, K275A, A204G, V205A and K206A (mut 7); c. K57A, deltaG272, K172N, F173L, T174A and V175A (mut 8); or d. K57A, deltaG272, A204G, V205A and K206A (mut 9); or a recombinant variant Phleum pratense pollen allergen 5 (Phl p 5) or Poa pratense pollen allergen 5 (Poa p 5) protein allergen corresponding to said recombinant variant of Lol p 5. (62) Divided Out of 531882