PRIMER COMBINATION FOR DIAGNOSING TOBACCO RATTLE VIRUS AND USES THEREOF

摘要

PURPOSE: A primer set for diagnosing tobacco rattle virus(TRV) and a method for diagnosing TRV using the same are provided to accurately diagnose TRV infection from Solanaceae seed or plant. CONSTITUTION: A primer set for diagnosing TRV comprises a forward primer selected from sequence numbers 1, 2, 3, 4, 5 and 6, and reverse primer selected from sequence numbers 9, 10, 11, 12, 13, 14 and 15. The primer set for diagnosing TRV comprises a primer set of sequence number 3 and 1 and primer set of sequence numbers 1 and 14. A method for diagnosing TRV comprises: a step of isolating total RNA from a plant sample; a step of amplifying a target sequence by RT-PCR; and a step of detecting PCR product.