Process for preparing highly unsaturated fatty acid and lipid containing highly unsaturated fatty acid

摘要

Methods are disclosed in which a microorganism belonging to the genus mortierella is cultivated to produce a lipid containing the desired polyunsaturated fatty acids, for example arachidonic acid, dihomo-³-linolenic acid and/or eicosapentaenoic acid. The carbon source concentration in the culture medium is at least 4% by weight, and the mortierella microorganism is selected for resistance to high carbon source concentration. Lipid or polyunsaturated fatty acid is recovered from the culture. Culturing microorganism for about a week can give at least about 7g/L of the highly unsaturated fatty acid.