| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide a means for simply and rapidly detecting or identifying Phormidium tenue and a means for enabling determination of group level and stock level of Phormidium tenue which have been impossible by microscope observation in a conventional method. <P>SOLUTION: Sequences of 16S-rDNA of Phormidium tenue were compared and attention was focused on the sequence having high specificity and various forward primers and reverse primers are designed and PCR was carried out by using any of these primers with 16S-rRNA gene of blue algae generating fungal odor in a sample to be tested as a template and whether PCR product exists or not was investigated. As a result, a primer capable of accurately detecting gene of Phormidium tenue in high sensitivity and high reproducibility was found. Examination by nucleic acid amplification method using the primer can specify Phormidium tenue in a level of strain or group. <P>COPYRIGHT: (C)2005,JPO&NCIPI |
