| 摘要 |
<p>An adult mammalian stem cell is provided which is pluripotent and non-teratogenic in immune deficient animals. The mammalian stem cell is obtainable by culturing olfactory epithelium in the presence of one or more of FGF2, FGF9, SCF, LIF, TGF- ß2 and BMP-4. The mammalian stem cell expresses low levels of Sox2 and Oct4 mRNA and higher levels of SP1 and ANP32A mRNA. The mammalian stem cell expresses one or more proteins such as OCT-3./4, SOX2, NANOG, KLF4, MVH, KIT, NESTIN and also selected CD antigens. The mammalian stem cell may be useful in compositions and methods for treating diseases or conditions responsive to cell-based therapies, including but not limited spinal repair, heart muscle regeneration, skin repair, bone-marrow transplantation and repair of neural degeneration. The mammalian stem cell and non-human mammals generated therefrom may be useful in drug screening, disease modelling and identifying cell differentiation pathways.</p> |
