发明名称 |
METHOD OF OBTAINING RECOMBINANT MUTANT CYTOCHROME C |
摘要 |
FIELD: chemistry; biochemistry. ^ SUBSTANCE: invention pertains to bioengineering. In particular, the invention relates to method of obtaining recombinant mutant horse cytochrome c. This method is realised by introduction of K27E/E69K/K72E/K86E/K87E/E90K or K8E/E62K/E69K/K72E/K86E/K87E or K8E/K27E/E62K/E69K/K72E/K86E/K87E/E90K mutations through site-directed mutagenesis into the horse cytochrome c gene which is contained in pBPCYCS/3 plasmid DNA. Further, the Escherichia coli JM-109 strain of the obtained recombinant plasmid DNA is transformed and the target protein is expressed and introduced through cation-exchange and adsorption chromatography. ^ EFFECT: invention enables use of recombinant mutant horse cytochrome c as a test system for measuring the rate of generation of superoxide in membrane preparations. ^ 3 dwg, 5 ex |
申请公布号 |
RU2391406(C2) |
申请公布日期 |
2010.06.10 |
申请号 |
RU20080128521 |
申请日期 |
2008.07.15 |
申请人 |
UCHREZHDENIE ROSSIJSKOJ AKADEMII NAUK INSTITUT BIOORGANICHESKOJ KHIMII IM. AKADEMIKOV M.M. SHEMJAKINA I JU.A. OVCHINNIKOVA RAN |
发明人 |
CHERTKOVA RITA VALER'EVNA;PEPELINA TAT'JANA JUR'EVNA;OSTROVERKHOVA TAT'JANA VLADIMIROVNA;DOLGIKH DMITRIJ ALEKSANDROVICH;VINOGRADOV ANDREJ DMITRIEVICH;GRIVENNIKOVA VERA GEORGIEVNA;KIRPICHNIKOV MIKHAIL PETROVICH |
分类号 |
C07K14/80;C12N1/21;C12N15/12;C12P21/02;C12R1/19 |
主分类号 |
C07K14/80 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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