发明名称 |
Process for obtaining recombined nucleotide sequences in vitro, libraries of sequences and sequences thus obtained |
摘要 |
Ligation-mediated method of recombining polynucleotides in vitro. Polynucleotides from a library are fragmented and the fragments are hybridized to an assembly template. The hybridized fragments are iteratively re-hybridized and ligated until the ends of the hybridized fragments are adjacent to the ends of other hybridized fragments on the assembly template. A final ligation produces recombined polynucleotides.
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申请公布号 |
US7718786(B2) |
申请公布日期 |
2010.05.18 |
申请号 |
US20050143455 |
申请日期 |
2005.06.03 |
申请人 |
PROTEUS SA |
发明人 |
DUPRET DANIEL;MASSON JEAN-MICHEL;LEFEVRE FABRICE |
分类号 |
C07H21/02;C12N15/10;C12Q1/68 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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