CLONING OF ANTIBODY GENES USING UNIQUE RESTRICTIONS SITES

摘要

The present invention relates to a nucleic acid construct encoding an immunoglobulin heavy and/or light chain, wherein said nucleic acid construct comprises one or more non-naturally occuring restriction sites and wherein said non-naturally occuring restriction sites are incorporated at one or more of the following positions: i) at or within about 25 nucleotides of the 3' end of the leader sequence of said immunoglobulin heavy and/or light chain; and/or ii) between the variable and constant regions of said immunoglobulin heavy and/or light chain; and/or at or within about 25 nucleotides of the 3' end of the variable region of said immunoglobulin heavy and/or light chain; and/or iii) at or within about 25 nucleotides of the 3' end of the stop codon of the constant region of said immunoglobulin heavy and/or light chain; and/or iv) at or within about 25 nucleotides of the 5' end of the leader sequence of said immunoglobulin heavy and/or light chain.