| 摘要 |
The invention relates to a method of identifying and/or differentiating cyanophyta comprising the steps of, (a) enzymatically digesting at least a first genomic DNA from a cyanophyta isolate to be identified or differentiated, with at least two different restriction enzymes, into restriction fragments, wherein a first restriction enzyme is a four cutter and a second enzyme is a six cutter, (b) ligating double stranded nucleic acid oligonucleotide adaptor fragments to the nucleic acid ends of the restriction fragments created in step (a), (c) pre-amplifying the fragments using pre-amplification primers which are substantially complementary to at least part of the restriction sites generated in step (a) and substantially complementary to at least part of the double stranded nucleic acid oligonucleotide adaptor fragments ligated in step (b), (d) selectively amplifying the fragments generated after step (c) by using (i) least one pre-amplifying primer and (ii) at least one selection primer which is substantially complementary to at least part of the restriction sites generated in step (a) and substantially complementary to at least part of the double stranded nucleic acid oligonucleotide adaptor fragments ligated in step (b) and which comprises at least two additional nucleotides at its 3'-OH. The invention also relates to kits. |
