| 摘要 |
Provided are highly productive mutant alpha -amylases which are derived from an alpha -amylase having an amino acid sequence represented by SEQ ID No. 1 or 2 or showing at least 60% homology thereto and are constructed so that a specific amino acid residue taking part in productivity is deleted or substituted with another amino acid residue, a gene encoding the mutant alpha -amylase, vector, transformant cell, a method for producing the mutant alpha -amylase, which comprises cultivating the transformant cell, and a detergent composition containing the mutant alpha -amylase. According to the present invention, alpha -amylases can be produced at a high yield from a recombinant microorganism, making it possible to drastically reduce a cost of their industrial production. This leads to production increase of liquefying alkaline alpha -amylases having heat resistance, chelating agent resistance and oxidant resistance and being useful as enzymes for a detergent. |
