A COMPOSITION FOR ACCELERATING MINERALIZATOIN OF TEETH ENAMEL CONTAINING THE RECOMBINANT ODAM PROTEIN

摘要

PURPOSE: A composition containing ODAM recombinant protein is provided to promote mineralization of enamel and evaluate expression level of the ODAM gene. CONSTITUTION: A composition for promoting mineralization of enamel contains an ODAM recombinant protein. A method for producing the ODAM recombinant protein comprises: a step of culturing E.coli(DE3) transformed with pLysS(pRSET-ODAM); a step of isolating a protein from E.coli(DE3); and a step of purifying ODAM protein using His-Tag protein through FPLC. A method for evaluating overexpression of ODAM gene comprises: a step of collecting ameloblast from teeth; a step of measuring mRNA expression level of gene of ODAM, MEF2, aurora kinase A, BMP receptor IB or EF-hand calcium binding protein, and GAPDH from the ameloblast; and a step of comparing mRNA expression level between the ameloblast and normal ameloblast based on mRNA expression level of GAPDH gene.