| 摘要 |
<p>PURPOSE: A method for detecting lactobacillus in Kimchi fermentation through multiplex PCR is provided to accurately and quickly detect grouping pattern of lactobacillus and recue time and cost. CONSTITUTION: A primer set for detecting lactobacillus in Kimchi fermentation using multiplex PCR comprises a primer pair of sequence number 1 and 2, 3 and 4, 5 and 6, 7 and 8, 9 and 10, 11 and 12, 13 and 14, 15 and 16, 17 and 18, or 19 and 20. A method for detecting lactobacillus in Kimchi fermentation comprises: a step of making a primer set which is specific to lactobacillus relating to Kimchi fermentation; a step of collecting a sample from Kimchi fermented 4°C~25°C in a specific time interval; a step of measuring change of strain number; a step of irritation; a step of confirming 10 kinds of lactobacillus by performing m-PCR; a step of confirming with a 16S rDNA(ribosomal DNA); and a step of detecting final m-PCR.</p> |
