| 摘要 |
<PICT:1092059/C2/1> The invention relates to anthelvencin, an antibiotic having anthelmintic, antibacterial and antifungal activity, and characterized in that the 2-naphthalenesulphonate addition salt is a white, crystalline powder of m. pt. 181-183 DEG C., soluble in water, methanol and benzyl alcohol, but insoluble in ether, chloroform or benzene; free base has pK1a of 9.58 and 11.95; molecular wt. of above salt 847; salt has empirical formula C39H44N9O9S2 and contains C = 54.78% H = 5.23%, N = 14.66%, O = 17.38% and $ = 7.41%; I.R. spectrum as in Fig. 1; specific rotation + 11.7 (95% ethanol); U.V. max. <FORM:1092059/C2/1> 330; positive in Ehrlich test. The antibiotic is produced by submerged aerobic cultivation of Streptomyces venezuelae A.T.C.C. 14583-5 or mutants thereof. Anthelvencin is recovered from the clarified broth by adsorption on an acidic ion-exchange resin followed by elution by means of an aqueous acidic water-miscible solvent such as acetone. Impurities may be removed on a magnesium silicate adsorbent at pH 5.5 followed by addition of NaCl to effluent, extraction with benzyl alcohol and addition of acetone to produce anthelvencin HCl. Separation of a salt of anthelvencin into components A and B is by fractional chromatography on pretreated cellulose powder using the system n-butanol, pyridine, acetic acid and water 15:10:1:12. Anthelvencin forms salts with inorganic and organic acids, e.g. p-bromo-salicylic acid, picric acid, m-nitrobenzoic acid, flavianic acid, p-nitrophthalic acid, salicylic acid and p-(2-hydroxy-1-naphthylazo) benzene sulphonic acid.ALSO:An anthelmintic composition comprises an animal feed and the antibiotic anthelvencin (components A and B) or an acid addition salt thereof. Other anthelmintics such as piperazine and hygromycin B may be present. |
