| 摘要 |
<p><P>PROBLEM TO BE SOLVED: To provide a method for the development of mutant animals, (including genetically engineered animals and those carrying spontaneous mutations), as human disease models, in particular to provide an integrated technology, (including rigorous specifications and quality control), for the development of animal models that can serve as a living assay system, useful in biomedical research and in the development of human therapeutics. <P>SOLUTION: The method of establishing a mutant animal line includes the steps of (a) inducing superovulation in a sexually immature mutant founder animal (G0), (b) fertilizing the superovulating sexually immature mutant founder animal, (c) delivering a first generation mutant animal (F1) upon completion of the gestation period, (d) confirming stability of the mutation, genotype, and identity of genetic background in the first generation mutant animal, and, if desired, (e) repeating the steps (a) to (d) with one or more further generations of mutant animals. <P>COPYRIGHT: (C)2010,JPO&INPIT</p> |
