摘要 |
<P>PROBLEM TO BE SOLVED: To provide methods, reagents and kits for amplifying polynucleotide sequences of interest in a multiplex fashion. <P>SOLUTION: Provided are methods, reagents and kits for carrying out a variety of assays suitable for analyzing polynucleotides or samples. As the variety of assays, an amplification step performed in a multiplex fashion is included. Also provided are methods for analyzing and improving the efficiency of amplification and methods for carrying out gene expression analysis. According to one embodiment of the methods, one or more polynucleotides are amplified by using multiple amplification primer pairs or sets, each of the multiple amplification primer pairs or sets is suitable for, or acts on amplifying a polynucleotide sequence of separate interest. <P>COPYRIGHT: (C)2010,JPO&INPIT |