TEST-SYSTEM FOR DIAGNOSING PROSTATE CANCER AND A PROSTATE CANCER DIAGNOSIS METHOD

摘要

The invention relates to medicine, in particular to oncology and molecular biology and can be used for the earlier diagnosis of the prostate pathologies, including prostate cancer by means on a non-invasive method. The invention is characterised in that recombinant protein hepsin, produced from a pHPNTM-A N°B- 100978 producer strain, is used as an agent for verifying the reaction quality in the test system, in that 0.1M tris-imidazole is used as a reaction buffer for reaction verification and setting, in that tetrapeptide L-lysine P2 ?3 L-arginine, wherein P2 - L-asparagine or L-leycine or L-threonine, P3 - L-lysine or L-glutamine conjugated with chromogen, is used as a substrate, in that the prostate cancer presence and the stage thereof are ascertained according to the hepsin Ao activity in nmol/l·min which is determined according to the mean value ???/minute of the change rate per minute of the density of a treated urine sample containing the substrate and the reaction buffer added thereto, wherein the test results obtained when the activity of a check hepsin Ak, verified with the aid of the same test-system, differs from the reference values Ae by more than 10%, were excluded