发明名称 |
Charge switch nucleotides |
摘要 |
The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
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申请公布号 |
US7625701(B2) |
申请公布日期 |
2009.12.01 |
申请号 |
US20050154419 |
申请日期 |
2005.06.15 |
申请人 |
PACIFIC BIOSCIENCES OF CALIFORNIA, INC. |
发明人 |
WILLIAMS JOHN G. K.;BASHFORD GREGORY;CHEN JIYAN;DRANEY DAN;NARAYANAN NARA;REYNOLDS BAMBI;SHEAFF PAMELA |
分类号 |
C12Q1/68;G01N21/64;C07B61/00;C07H19/04;C07H19/10;C07H19/20;C07H21/00;C07H21/02;C12N15/09;C12P19/34;C12Q1/00;C12Q1/42;C12Q1/44;C12Q1/48;G01N27/447;G01N33/483;G01N33/53 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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